Molecular detection and genetic diversity of virulent Aeromonas hydrophila (Chester, 1901) Stanier, 1943 isolates from raw and processed fish

Aeromonas hydrophila is a mesophilic motile aeromonad present ubiquitously and causes high number of fish mortalities particularly in India along with various intestinal extraintestinal diseases humans. In this study, total 88 samples their processed products were screened by PCR targeting species-specific 16S rRNA gene, the virulent genes such as aerA, hly ahh1. The findings study showed that 36.36 % harboured A. which 51.61 %, 71.87 78.12 isolates possessed hly, ahh1, respectively. phylogenetic analysis using AP-PCR assay suggested five different clusters organisms prevalent among degree (> 95.00 %) lineage. conclusion, it may be presumed raw pathogenic serve reservoir/ source infection to human beings.